Písek L., Šoch M., Lukešová D.
Abstract
The project was aimed at determining physiological values in activity o ALP (EC 3.1.3.1) in blood plasma of cattle
from organic and conventional stocks according to stage of pregnancy. Four blood samples were collected from the
two stocks over a period of two years. Blood was collected in the morning and in the afternoon from vena jugularis
with one-shot needles into test tubes with heparin. A total of 45 and 43 blood samples were collected in stocks A and
B, respectively. The blood serum was obtained by centrifuging blood samples for 10 min at 1500 rpm. To determine
ALP [µkat.l-1] activity in blood plasma, Bio-La-Tests (Lachema Brno) and Specol spectrophotometer (Carl Zeiss,
Jena) were used. Statistically significant differences in ALP activity between stocks A and B were found (P < 0.05).
In stock A, statistically significant differences were found between animals in the second 140 days of gestation and
non-pregnant animals (P < 0.01), and animals after calving. Differences between stocks A and B in animals in the
second 140 days of gestation were also found (P < 0.05).
Key words:
cattle, organic stock, conventional stock, blood plasma, alkaline phosphatase, gravidity