DVOØÁÈEK V., ÈEPKOVÁ P.
Abstract
The role of state inElectrophoretical study of protein fractions (albumins + globulins, prolamins and sum of glutelins) in SDS-PAGE
conditions and their quantification were tested in eight varieties of common buckwheat and in two tartary buckwheat
accessions. The electrophoretical visualization showed as a main fraction albumins + globulins with high inter- and
intra-varietal polymorphism in tested varieties. Tartary buckwheat accessions showed completely uniform electrophoretical
spectra. A low appearance of prolamins was confirmed in all tested varieties (inclusive tartary buckwheat accessions).
Spectrum of soluble glutelins in common buckwheat was characterized by lower frequency and intensity of protein bands.
In both tartary buckwheat accessions were quite comparable with fraction of albumins + globulins. The overlapping zones
were presented in all varieties especially between 20 - 50 kDa. The statistically significant higher values of crude
protein were obtained in common buckwheat varieties. All tested common buckwheat varieties showed almost two times
higher content of albumins + globulins (5.24-7.51% in d.m. with 43.57-54.37% proportion in crude protein)
than accessions of tartary buckwheat (3.36-3.57% in d.m. with 27.84-28.21% proportion in crude protein).
Prolamins content was very low in all common and tartary buckwheat. Both tartary buckwheat accessions
showed about 20% higher content of sum of glutelins in crude protein (68.38% and 69.58%) than tested common
buckwheat varieties (44.15 - 53.66%).
Key words:
Common buckwheat, Fagopyrum esculentum Moench, Tartary buckwheat, Fagopyrum tataricum Gaertn., Protein fractions, SDS-PAGE,