EYASU ABRAHA , MIROSLAV BECHYNĚ, MIROSLAV KLÍMA, MIROSLAVA VYVADILOVÁ
Abstract
Microspore culture protocol has been modified to produce satisfactory amount of embryos from six genotypes of Brassica carinata. The highest frequency of cell division and embryo yield was obtained from 2.5 mm to 3.25 mm long buds in cultivar Dodolla, whereas other genotypes of B. carinata utilized in the experiment were favourable when microspores from 3 - 3.5 mm long buds were cultured under continuous 30°C temperature treatment. Significant difference in frequency of microspore embryogenesis was also determined between genotypes. Cultivation of microspores in the liquid NLN medium with 0.05 mg/l colchicine for 18 hours was more efficient for the embryo production than the control.
Key words:
Brassica carinata; haploids; doubled haploids; microspore embryogenesis